Announcements and Other Useful Info

---

WHAT'S GOING ON IN LAB TODAY? Google Forms are due at 12pm on lab days
Link to Google Docs Biol 322 Form

---

Required Reading Assignments:
Deaf by Design
Identification of SLEEPLESS, a Sleep-Promoting Factor
Super Bugs on the Hoof
Triple-threat Microbe Gained Powers from Another Bug
Weapons of Microbial Drug Resistance Abound in Soil Flora
Trench Warfare in Battle with TB
Inspired Choices

Optional Reading Assignments:
Phage on the Rampage
Humans as the world's greatest evolutionary force

---

Follow this link to an interesting series of articles on human genetics: The DNA Age

---

---

COURSE INFORMATION
Biol 322 Timetable: Updated 11/4/12
Biol 322 Course Syllabus
Summary of Point Allocations for Course
Biol 322: Info on lab notebooks
A good example of a Lab Notebook Table of Contents
Link to Google Docs Biol 322 Form
Biol 322 Homework Assignments & Study Problems for quizzes

---

Biol 322 Course Materials Including Student Data Organized by Date of Distribution

---

LAB EXERCISES:
Exploring Biological processes using Caenorhabitis elegans: Mendel Revisited

---

Arabidopsis Part 1: Phenotypic and genotypic analysis of F2 segregants from aha/+ heterozygotes
Arabidopsis Part 2: Phenotypic and genotypic analysis of F2 segregants from aha/+ heterozygotes
aha gel images
Hazards of Ultraviolet Light
Useful information about Ethidium Bromide courtesy Wikipedia
Ethidium Bromide: Hazards & Cautions -courtesy UC Berkeley EH&S
Instructions for aha Lab Report (due Nov 6)
aha gel images
Class Data: aha genotypes

---

Genes we Share and Exploring Online Resources (see also lecture link below)
Introduction to Cancer Genetics

---

Road map to mutagenesis experiments
An investigation of mutagenesis using the model organism Escherichia coli: Part 1
Answers to Dilution Problems in Appendix D
Mutagenesis Part 2: preparation of genomic DNA from rifR strains and isolation of lac- reverse mutations
DNeasy Kit Handbook
Mutagenesis Part 3: Using public databases to gather information required for our sequence analysis of the rifR mutant lines
Work-up of Mutation Frequency Data
Updated Class data for Mutagenesis lab as pdf file
Updated Class data for Mutagenesis lab as docx file
Mutation lab gel images
Mutagenesis Part 4: Analyzing rpoB sequence data from rifR mutant lines
Supplement to Part 4: Info on sequence display conventions and detailed instructions on translating your sequence files
Sequence Files in a WORD document
Work-up of rpoB sequence data from rifR mutant lines

---

---

Bacterial Genetics: F' X F- cross
Class data (and followup) for F' X F- cross

---

Exploring Complementation with the jewel wasp Nasonia vitripennis
More Info about the Nasonia experiment including life-cycle and developmental stages info

---

Reverse Genetics in C. elegans: RNAi knockdown of specific genes

---

---

LECTURE MATERIAL:
10/1/12 Lecture: Introduction to Biol 322 and Model Organisms
10/9/12 Lecture: Introduction to Chi-square goodness-of-fit test
10/15/12 Lecture: Background on aha-3 and T-DNA mutations and Direct Detection ofGenotype
10/22/12 Lecture: Using E. coli as a model to study mutation rates
10/30/12 & 11/6/12 Lecture: Antibiotic Resistance Mechanisms & Horizontal Gene Transfer
11/5/12 Lecture: Using Forward Genetics to Analyze Biological Processes
11/15/12 Lecture: Complementation and the non-traditional model Nasonia vitripennis
11/19/12 Lecture: Loose ends on Horizontal Gene Transfer and Antibiotic Resistance
11/26/12: Introduction to RNAi
11/27/12: Mini lecture on dideoxysequencing

---

---

USEFUL WEB LINKS:
Model Organism Guide
Model Organism Web Sites
NCBI: Outreach and Education
LINKS to Textbooks:
Genomes by TA Brown, 2nd edition
An Introduction to Genetic Analysis by A Griffith et al., 7th edition
Molecular Biology of the Cell by B Alberts et al., 4th edition

---

Carol Trent's Biology Courses