Dr. Craig Moyer's

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Biology 324 - Methods in Molecular Biology

Laboratory Outline

MMB Course Syllabus UPDATED 10/15/04
Link to Text Book

Weekly Laboratory Handouts & Required Information

Week 1 & 2

Basic Tools for Molecular Biology: Reading Assignment
Gel Electrophoresis: Reading Assignment
Manipulation of DNA: Reading Assignment
Restriction Digestion Tables: Handout
Restriction Digestion Rules: Handout
Tips & Tricks for Agarose Gels: Handout
Dilutions and Solutions: Handout (Due next week)
Restriction Digestion of Plasmid DNA: Laboratory Excercise

Week 3

QIAprep Plasmid Protocol: Laboratory Excercise
QIAprep Plasmid HandBook: Reference Only
How to guide for DNA quantification: Laboratory Excercise

Week 4

Introduction to PCR (part 1): Reading Assignment
Polymerase Chain Reaction: Laboratory Excercise

Week 5

Purifying and Analyzing gDNA part 1: Reading Assignment
Purifying and Analyzing gDNA part 2: Reading Assignment
The Isolation and Purification of gDNA: Laboratory Excercise

Week 6

Applications for PCR (part 2): Reading Assignment
Polymerase Chain Reaction II: Laboratory Excercise

Weeks 7 & 8

Intro of DNA into Living Cells (aka Cloning!)
Suicide Plasmid Vector: Handout
TOPO TA cloning for Sequencing Manual: Laboratory Excercise
M13 Screen PCR Worksheet: Handout

Week 9

QIAprep Plasmid Protocol: Same as Above (Week 3)
PCR Sequencing: Reading Assignment
Fluorescence-based DNA Sequencing: Reading Assignment

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